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Changes in [Ca 2+ ] i response of individual Jurkat cells to nanosecond pulsed electric fields (nsPEFs) of 60 ns and field strengths of 25, 50, and 100 kV/cm were investigated. The magnitude of the nsPEF-induced rise in [Ca 2+ ] i was dependent on the electric field strength. With 25 and 50 kV/cm, the [Ca 2+ ] i response was due to the release of Ca...
We have developed a Blumlein line pulse generator which utilizes the superposition of electrical pulses launched from two individually switched pulse forming lines. By using a fast power MOSFET as a switch on each end of the Blumlein line, we were able to generate pulses with amplitudes of 1 kV across a 100-Omega load. Pulse duration and polarity can be controlled by the temporal delay in the triggering...
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