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Acridine orange test (AOT) is a simple microscopic procedure based on acid conditions to denaturant DNA followed by staining with acridine orange. The AOT measures the metachromatic shift of AO fluorescence from green (native DNA) to red (denatured DNA). Acridine Orange fluoresce green when it binds to native DNA and red when it binds to the fragmented DNA. Many authors observed that ≥50% green fluorescence...
Objective The purpose of this study was to determine and compare the concentration of reactive oxygen species (ROS) and total antioxident (TAS) in seminal plasma of IVF (in vitro fertilization) and ICSI patients, to establish their effect on sperm quality (count, vitality, HOS, morphology, maturity, DNA strand breaks) and assess the fertilization potential of spermatozoa and IVF/ICSI outcome. Method...
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