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The production of a mutant green fluorescent protein (S65TGFP), controlled by the maltose inducible glucoamylase promoter, was followed in situ in fed-batch cultures of recombinant Aspergillus niger using multi-wavelength fluorescence spectroscopy. Disturbance of quantitative product analysis by interfering fluorescence signals was resolved by using a set of defined combinations of excitation and...
Optical sensor systems are of increasing interest in bioprocess monitoring, because they are very sensitive, specific and noninvasive. Recent developments in optical-density probes, in situ microscopy, optical biosensors, optical-fiber sensors and infrared and fluorescence sensors demonstrate the growing importance of optical sensor systems. The possibility of continuous optical bioprocess monitoring...
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