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Alkaline phosphatase catalyzes the phosphates hydrolysis and ester transfication under the presence of external alcohol. This enzyme contains two Zn(II) ion and one Mg(II) ion at the active site, and it is believed that the key intermediate Zn(II)-OH - is afforded by the deprotonation of the Zn(II)-bound water. While the metal hydroxy moiety can be the good candidate for the nucleophilic...