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We have isolated a prostate-specific gene (NKX3.1) in humans that is homologous to theDrosophilaNK homeobox gene family. Northern blot analyses indicate that this gene is expressed at high levels in adult prostate and at a much lower level in testis, but is expressed little or not at all in several other tissues. In an androgen-dependent prostate carcinoma line, LNCaP,NKX3.1mRNA is expressed at a...
We have compared the regulation of the human metallothionein (MT)-IIA gene by the cytokines tumour necrosis factor-α (TNF) and interferon β (IFN-β) in human fibroblasts. Both TNF and IFN-β induced MT-II mRNA rapidly, but stimulation by TNF was more sustained. The effects of TNF and IFN-β were further distinguished by the action of the protein synthesis inhibitor cycloheximide, which reduced MT-II...
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