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The pe38 gene product of Autographa californica M nucleopolyhedrovirus (AcMNPV) has been shown to be involved in transcriptionally transactivating viral genes and augmenting viral DNA replication in transient assays. To assess the role of pe38 during infection, we generated a knockout virus, Δpe38-E9/E9, in which the pe38 open reading frame was replaced with that of the green fluorescent protein....
Autographa californicaM nucleopolyhedrovirus carrying a lacZ reporter gene was used to study pathogenesis and developmental resistance in fifth instarHeliothis virescens.Compared to fourth instar larvae, the progression of infection proceeded much more slowly in fifth instar larvae, and developmental resistance in orally inoculated larvae was much more sudden and severe. The resistance occurred within...
Previous reports have shown that the stilbene-derived optical brightener M2R increases mortality and hastens death of larval lepidopterans orally challenged with baculoviruses. The mechanism of viral enhancement, however, has not been elucidated. We used a reporter gene recombinant ofAutographa californicaMNPV (AcMNPV-hsp70/lacZ) to investigate the enhancing effects of M2R on pathogenesis, mortality...
We compared early viral pathogenesis and dose mortality relationships for larvae of two highly susceptible hosts, Trichoplusia ni and Heliothis virescens, using a construct of AcMNPV containing the lacZ reporter gene. Larvae were inoculated either as newly molted fourth instars (40) or 15 hr after the molt (415). In 40-inoculated larvae, first lacZ expression was detected in the midgut epithelium...
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