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Polyhydroxyalkanoates (PHAs) are gaining popularity as a viable biological alternative to petroleum-derived polyolefin plastic materials, especially because their material properties can be tuned through monomer composition and configuration, an uncommon phenomena in enzymatic polymer products. Because the native function of these polymers is chemical energy storage, the metabolic synthesis routes...
The O-linked β-N-acetylglucosamine (O-GlcNAc) post-translational modification is an important, regulatory modification of cytosolic and nuclear enzymes. To date, no 3-dimensional structures of O-GlcNAc-modified proteins exist due to difficulties in producing sufficient quantities with either in vitro or in vivo techniques. Recombinant co-expression of substrate protein and O-GlcNAc transferase in...
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