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Stepwise synthesis of hydrophobic peptides frequently yields deletion products, which often require extensive purification and identification. We synthesized a new transient-biotinylation reagent 9, which was conveniently used in affinity Chromatography of crude products ( exemplified by 11-I and 11-II), to afford pure peptides (15-I and 15-II, respectively). Unlike conventional affinity Chromatography,...
The ability of reduced peptide bond analogues of gastrin releasing peptide (GRP) to antagonize small cell lung cancer (SCLC) GRP receptors was investigated. BW462U89, BW1023U90, BW2123U89 and BW2258U89 inhibited binding of ( 125 I-Tyr 4 )BN to NCI-H345 cells with IC 50 values of 5, 6, 140 and 10 nM respectively. The GRP analogues had no effect on basal cytosolic...
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