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A simple assay format was developed for the direct detection ofC. trachomatisrRNA utilizing ligation of recombinant MDV-1 probe RNA fragments hybridized to 23S rRNA after capture and release from a solid support. Assay background (equivalent to 104targets) was suppressed by blocking sequences in the 5′ MDV reporter probe fragment complementary to the 3′ fragment by prehybridization of a DNA oligonucleotide...