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Trypanosoma cruzi, the etiological agent of Chagas disease, is an adequate model for studies on the evolution of signal transduction pathways. These pathways involve molecular entities such as membrane receptors, transduction G proteins, protein kinases and second messengers (Ca 2+ , cyclic AMP, cyclic GMP, nitric oxide). In this article, Mirtha M. Flawia, Maria T. Tellez-Inon and Hector...
Trypanosoma cruzi epimastigote forms showed a tightly bound Ca 2+ -calmodulin-dependent protein kinase activity, which could be partially extracted from membranes and axonemes. The enzyme is constituted by subunits which were autophosphorylated in the absence of exogenous substrates. An antibody against CaM kinase II recognized a Ca 2+ - or Ca 2+ -CaM-dependent...
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