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Background Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics in terms of average plasmid copy number (PCN) and stability are rarely known. The crucial factor determining the PCN is the replication system; most replication systems in use today belong...
Plasmids are circular DNA molecules that are used as gene vectors in countless applications. They are easy to manipulate and transfer, but can also vary considerably in copy number between individual cells. In this article, a model expression system with a large amount of heterogeneity is presented. We used flow cytometry and digital PCR to count the number of plasmids in sorted sub-populations. Finally,...
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