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The ibuprofen primary binding site FA3–FA4 is located in domain III of human serum albumin (HSA), the secondary clefts FA2 and FA6 being sited in domains I and II. Here, the thermodynamics of ibuprofen binding to recombinant Asp1–Glu382 truncated HSA (tHSA)–heme‐Fe(III) and nitrosylated tHSA–heme‐Fe(II), encompassing domains I and II only, is reported. Moreover, the allosteric effect of ibuprofen...
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