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Novel sulfur-linked squaryl group-containing glutamate analogs were synthesized via addition and mono-substitution reactions of thiols to t-butyl methyl squarate (BMSQ: 7) in two steps. A glutamate analog prepared from Boc-l-Cys showed a potent binding affinity to KA/AMPA receptors.
A cell wall lytic enzyme of Chlamydomonas reinhardtii has been purified and identified as a single glycopolypeptide subunit of 62 kDa by SDS-polyacrylamide gel electrophoresis. It is released into culture medium by mating gametes as a large aggregate of subunits. The purified enzyme shows a pH optimum at about 7.5 and 35 o C. Metal ion chelators and SH-blocking agents inhibit the activity...
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