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Cells of the aerobic denitrifier and heterotrophic nitrifier Thiosphaera pantotropha and of the traditional denitrifierParacoccus denitrificans were immobilized in a 1.5 mm thick agar layer (biofilm) and submersed in liquid medium. A combined microsensor for O 2 and N 2 O was used to record microprofiles of these two species in biofilms where the reduction of N 2 O was inhibited...