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We have studied the metabolism of xylose by Candida tropicalis in oxygen-limited chemostat. In vitro enzyme assays indicated that glycolytic and gluconeogenetic enzymes are expressed simultaneously facilitating substrate cycling. Enhancing the redox imbalance by cofeeding of formate increased xylose and oxygen consumption rates and ethanol, xylitol, glycerol and CO 2 production rates at steady...
Several approaches to reduce acetate accumulation inEscherichia colicultures have recently been reported. This reduction subsequently led to a significant enhancement in recombinant protein production. In those studies, metabolically engineeredE. colistrains with reduced acetate synthesis rates were constructed through the modification of glucose uptake rate, the elimination of critical enzymes that...
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