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We present the detailed imaging of structures and processes of the nematode Caenorhabditis elegans (C. elegans) using non-linear microscopy. Complementary information about the anatomy of the nematode was collected by implementing a combination of two photon excitation fluorescence (TPEF), second and third harmonic generation (SHG and THG) image contrast modes on the same microscope. Three-dimensional...
We present the detailed imaging of structures and processes of the nematode Caenorhabditis elegans (C. elegans) using non-linear microscopy (TPEF-SHG-THG). Consummative and specific information about the anatomy of the nematode was collected by implementing a combination of THG, SHG and TPEF image contrast modalities on the same microscope. Additionally, 3-D reconstruction of TPEF and THG images was...
In this paper an ideal approach for pulse characterisation within a multiphoton microscope is demonstrated. This methodology will be used for shaping the pulses with an spatial light modulators (SLM) and to study the influence of arbitrary phase profiles, specifically with large bandwidth pulses, on nonlinear imaging of the biological samples.
The generation of controlled broadband pulses using output pulsed of a Ti:sapphire laser traveling through a bending insensitive single mode fiber. A pair of prisms is used to control the chirp of the pulses from the fiber. A simple technique to increase the available bandwidth of the pulses from a Ti:sapphire laser is proposed. The pulses are characterized using a nonlinear microscope.
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