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We investigated the use of Eu 3+ chelate-labeled analogues of melanin-concentrating hormone (MCH) as ligands for both human MCH receptors (MCHR1 and MCHR2). The analogues employed were Ala 17 MCH, S36057 (Y-ADO-RC*MLGRVFRPC*W, where ADO=8-amino-3,6-dioxyoctanoyl and *=disulfide bond), and R2P (RC*MLGRVFRPC*Y-NH 2 ). The peptides were readily labeled on the α-amino...
The first direct and continuous fluorometric assay for monoamine oxidase B (MAO B) has been developed.E-2,5-Dimethoxycinnamylamine hydrochloride was designed and synthesized and was found to be an excellent substrate for MAO B (Km= 218 μM,Kcat= 435 min−1). This compound has an intense purple fluorescence when irradiated at λex= 343 nm (λem= 393 nm) in Tris buffer, pH 9.0, or sodium phosphate buffer,...
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