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The first direct and continuous fluorometric assay for monoamine oxidase B (MAO B) has been developed.E-2,5-Dimethoxycinnamylamine hydrochloride was designed and synthesized and was found to be an excellent substrate for MAO B (Km= 218 μM,Kcat= 435 min−1). This compound has an intense purple fluorescence when irradiated at λex= 343 nm (λem= 393 nm) in Tris buffer, pH 9.0, or sodium phosphate buffer,...