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The catalytic efficiency (k cat K m ) and the cleaved bond distribution for the nitrophenylated maltooligosaccharides, p-NPGlc n (2 n 7) hydrolysed by porcine pancreatic alpha-amylase isozymes I and II were determined. The subsite affinities (A i ) were calculated from the p-NPGlc n (4 n 7) hydrolysis data. Five subsites (-3 to 2) bind...