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Aggregation of the receptors with high-affinity for IgE (FcεRI) stimulates a variety of cellular responses, but excessive aggregation inhibits such responses. Actin filaments have been implicated in the inhibitory phenomenon because disrupting the filaments enhances the cellular reactions stimulated by the aggregated receptors. To clarify further the molecular mechanism and physiological importance...
Clustering the mast cell function-associated antigen (MAFA), a membrane glycoprotein expressed on 2H3 cells, by its specific monoclonal antibody G63 substantially inhibits secretion normally triggered by aggregating these cells' Type I Fc receptor (Fc RI). To explore possible MAFA-Fc RI interactions giving rise to this inhibition, we have studied by time-resolved phosphorescence anisotropy the...
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