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This work aims to identify ‘A’ and ‘B’ onion lines in Brazilian germplasm, associating marker-assisted selection (MAS) of cytoplasm type and the Ms locus with pollen viability analysis. MAS and pollen viability tests presented a complete agreement for the lines Alfa SF, BRS 367, BRS 367, Cascuda T7, Cascuda T5, EHCEB 20102017 and EHCEB 20142027. MAS was not validated by the pollen viability tests...