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The rapid loss in Survanta , a bovine lung extract based surfactant, activity upon storage at 30°C is investigated. During 3 months, the minimum surface tension as measured by both Wilhelmy plate and pulsating bubble methods rose by 8-9 mN/m. Additionally, a decrease in the in situ rat lung bioactivity of approximately 40% was observed. Disaturated phosphatidylcholine levels decreased while free...