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Binding of guanosine nucleotides to purified native and ADP-ribosylated wheat germ EF-2 was measured. Both forms of EF-2 bound [ 3 H]GDP to the same extent. [ 3 H]GDP binding to native but not to ADP-ribosylated EF-2 was reduced in the presence of GTP and ribosomes. Binding of [γ- 32 P]GTP to EF-2 was significantly reduced upon ADP-ribosylation. ADP-ribosylation almost...