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Adaptive mutation is an induced response to environmental stress in which mutation rates rise, producing permanent genetic changes that can adapt cells to stress. This contrasts with neo-Darwinian views of genetic change rates blind to environmental conditions. DNA amplification is a flexible, reversible genomic change that has long been postulated to be adaptive. We report the discovery of adaptive...
The majority of bacteria, which carry the N + –cI857[Ts]–cro − –O + –P + fragment of λ genome, are killed when derepressed by shifting from 30°C to 42°C. Among rare survivors, we observed a proportion of colony-forming units (cfu) that retained the typical imm λ −immunity phenotype when grown at 30°C; however, when shifted from 30°C to 42°C, they lost λ immunity...
The coordinate expression from induced λ prophages of p Lit -rexB-t Imm (late immunity transcription, LIT RNA) and p O -oop-t O (OOP RNA) has remained unexplained. The initial assigned sequence for p Lit bore no relationship to p O . We have identified two promoter sites for independent rexB transcription, denoted here p...
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