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This study reported an integrated microfluidic system for automating multiplex detection of allergy microarrays. Allergy has become a common disease worldwide. However, detection for allergy on microarrays requires tedious manual operation, well-trained technicians and a relatively labor-intensive process. In this study, an integrated microfluidic system combining the microfluidic techniques and the...
Time-resolved angled Fluorescence Laminar Optical Tomography (aFLOT) imaging system with 5 ms temporal resolution was developed to record 3D neural activities evoked by single whisker deflection in sensory and motor cortices of mice in vivo.
We applied time-resolved angled Fluorescence Laminar Optical Tomography (aFLOT) imaging system to record 3D neural activities evoked in the whisker-barrel system of mice in vivo, in response to single whisker deflection.
In this talk I will present an array of fluorescence molecular imaging technologies for multi-scale imaging from microscopic to macroscopic scales. Specific methods include planar fluorescence imaging for macroscopic samples, fluorescence laminar optical tomography (LOT) for mesoscopic (millimeter scale) samples, and two-photon fluorescence microscopy for intravital microscopic imaging. In addition,...
We combined voltage-sensitive dye imaging (VSDi) with gradient-index (GRIN) rod lens to study neural functions in mice vibrissae system. Neural activities evoked in the thalamic barreloids by single whisker stimulation were visualized in vivo.
We have developed a line-scanning angled fluorescent laminar optical tomography (LS-aFLOT) system. This system enables three-dimensional imaging of fluorescent-labeled stem cell distribution within engineered tissue scaffold over a several-millimeter field-of-view.
Theoretical analysis indicated FLOT resolution can be improved by angled incidence and detection. We developed an angled FLOT system and experimentally demonstrated its capability for depth-resolved fluorescence imaging in scattering medium.
We developed a co-registered optical coherence tomography (OCT) and fluorescence molecular imaging (FMI) system for simultaneous morphological and molecular imaging. This system enables real-time imaging of fluorescence-labeled gold nanoparticles and monitoring photothermal therapy.
We have developed a co-registered optical coherence tomography (OCT) and line-scanning fluorescence laminar optical tomography (FLOT) imaging system. This system enables simultaneous 3-D imaging of tissue morphology and molecular information 10-100 mum resolution and millimeter-scale imaging depth.
The objective of this study is to investigate the feasibility of imaging colon cancers using multi-modal optical imaging system combining optical coherence tomography (OCT) and fluorescence molecular imaging (FMI). This system enables simultaneous imaging of tissue morphology and molecular information at high resolution (10-20 mum) over a 3-5 mm field-of-view. OCT reveals the microstructures of normal...
We have developed a hybrid optical tomography system combing optical coherence tomography (OCT) and line-scanning fluorescence laminar optical tomography (FLOT). This system could be used for concurrent depth-resolved tissue-structural and molecular imaging.
We present a combined optical coherence tomography (OCT) and line-scanning fluorescence laminar optical tomography (FLOT) system for simultaneous 3-D morphological and molecular imaging with 10–100 µm resolution and millimeter-scale imaging depth. Co-registration on a capillary phantom with fluorescence dye Cy5.5 using the system has been demonstrated.
We have developed a co-registered optical coherence tomography (OCT) and laser scanning fluorescence molecular imaging system. This system enables simultaneous imaging of tissue morphology and molecular information at high resolution over a several-millimeter field-of-view.
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