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Lipoteichoic acids (LTAs) have the ability to form stable complexes with polysaccharides in ethanol solutions. When crude preparations of LTA from Bacillus subtilis and Streptococcus sobrinus were poured over a column of Sephacryl S-200 equilibrated with 80% ethanol, the LTAs bound strongly to the column. Elution of the LTA was brought about by distilled water. The affinity-purified LTA was able to...