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Aims/hypothesis Efficient islet isolation is an important prerequisite for successful clinical islet transplantation. Although progressively improved, islet yield and quality are, however, unpredictable and variable and require standardisation. Methods Since 1989 we have processed 437 pancreases using the automated method. The donor characteristics, pancreas procurement, and digestion and purification...
Binding of human preformed natural antibodies (PNAb) to xenogeneic antigenic determinants on porcine endothelial cells (EC) leads to hyperacute rejection, a phenomenon that characterizes the recognition of transplanted vascularized organs. A major epitope recognized on EC by human PNAb is the Gal α 1,3 Gal disaccharide, the expression of which is due to the presence of an α1,3 galactosyltransferase...
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