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In flowering plants, immotile sperm cells develop within the pollen grain and are delivered to female gametes by a pollen tube [1, 2]. Upon arrival at the female gametophyte, the pollen tube stops growing and releases sperm cells for successful fertilization [3]. Several female signaling components essential for pollen tube reception have been identified [4–11]); however, male components remain unknown...
In double fertilization, a reproductive system unique to flowering plants, two immotile sperm are delivered to an ovule by a pollen tube. One sperm fuses with the egg to generate a zygote, the other with the central cell to produce endosperm [1]. A mechanism preventing multiple pollen tubes from entering an ovule would ensure that only two sperm are delivered to female gametes. We use live-cell imaging...
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