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reg was originally identified a a gene expressed during the regeneration of insulin-producing pancreatic β-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic...
cDNA clones coding for glucagon were isolated from a chicken pancreas cDNA library, and the nucleotide and amino acid sequences were determined. The amino acid sequence of chicken glucagon was HSQGTFTSDYSKYLDSRRAQDFVQWLMST, which was contained in the 151-amino acid long precursor, being preceded by a signal sequence and an amino-terminal peptide (NH 2 -peptide) and followed by an intervening...
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