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Photoinactivation and reversion to tryptophan prototrophy were studied in four Escherichia coli strains with different repair deficiencies. Cells were irradiated with 222-nm wavelength UV emitted by an excimer lamp and with 254-nm wavelength UV emitted by a low-pressure mercury lamp.Strain DSM 9494 (trp − uvrA + ) turned out to be most resistant while the strain DSM 9495 (trp −...
Catalase (EC 1.11.1.6) isoforms CAT 2 through CAT 8 were purified from peroxisomes of sunflower (Helianthus annuusL.) cotyledons and photoinactivatedin vitro.Action and absorbance spectra between 380 and 727 nm wavelength showed most prominent maxima at 405 nm suggesting an inactivation mediated by light absorption of heme groups. First order kinetics of inactivation were observed for CAT 6 through...
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