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A method was developed for the enrichment and separation of proteins by an aqueous two-phase extraction system (ATPS) of PEG/(NH 4 ) 2 SO 4 combined with high-performance liquid chromatography (HPLC). The effects of the type and the concentration of inorganic salts, PEG molecular weight, pH value, temperature and NaCl on the formation of two phases and partition of cytochrome...
1-allyl-3-methylimidazolium ionic liquids were synthesized and polymerized respectively in-column or out-column as stationary phases in capillary gas chromatography. The influences of different polymerized precedure were studied. The results indicated that 1-allyl-3-methylimidazolium hexafluorophosephate ionic liquids possessed good column coating property and a chromatographic separation ability...
Molecular imprinting is a method to fabricate a polymeric material (molecularly imprinted polymer or MIP) capable of selectively recognizing template molecules. Molecular imprinting of small molecules has been studied widely. Less common, however, is the imprinting of biological macromolecules, including proteins, among which lysozyme is an important molecule in the food, pharmaceutical, and diagnostic...
Molecular imprinting, a templated polymerization technique, is a promising method to prepare a functional polymeric network for targeted binding of molecules in a selective recognition process. In this work, a silica-based (polysiloxane) polymer was utilized in a sol-gel process to fabricate a molecularly imprinted polymer (MIP). The manufactured MIP was imprinted with a template molecule, lysozyme...
As radio-labeled acetate is a good candidate to detect glial metabolism in brains and prostate cancer, ethyl and sodium [18F] fluoroacetates were utilized in clinical PET check. A simple and fast method for preparation of ethyl and sodium [18F]fluoroacetates using Explora FDG4 module has been established. By this development, it has become possible to obtain ethyl and sodium [18F] fluoroacetate in...
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