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We used the whole-cell configuration of the patch-clamp technique to investigate modulation of the voltage-dependent K + conductance of rat Leydig cells by intracellular pH, Hepes, and Na + . pH (range 6.8-7.4) has no effect on current. Removal of Na + (from 10 to 0 mM) increases the amplitude of K + current and abolishes the plateau effect on peak current. Reduction...
Voltage-clamped membrane currents have been investigated from whole-cell patch-clamp recordings performed on single Leydig cells isolated from the adult rat testis. Two outward membrane currents were evoked by depolarizing voltage steps. A potassium current was recorded in cells dialyzed with low (10 -9 -10 -8 M) calcium media. This current was decreased by TEA (10 mM). A...
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