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Two myrosinase isoenzymes (EC 3. 2. 3. 1) were extracted and purified by (NH 4 ) 2 SO 4 precipitation and DEAE-cellulose and Sephadex G-200 chromatography from radish root tissues with M r s 28 800 and 58 900. The most active one (120 U mg -1 ) was identified and characterized. The yield of the purified myrosinases was 21 mg (2398 U) of pure enzymes from...
The enzyme β-amylase (1,4-α-d-Glucan maltohydrolase, E.C. 3.2.1.2) was isolated and purified to homogenity from the extract of healthy radish roots (Raphanus sativus). The purification involved ammonium sulphate precipitation (100% saturation); DEAE-cellulose; hydroxylapatite and Sephadex G-200 chromatography. The purity and homogenity of the enzyme preparation were judged by gel filtration on Sephadex...
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