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New methods to manipulate gene and cell state can be used to engineer cell functionality, simplify quality assessment, and enhance manufacturability. These strategies could help overcome unresolved cell therapy manufacturing challenges and complement frameworks to design quality into these complex cellular systems, ultimately increasing patient access to living therapeutics.
We describe the use of a new baculovirus expression vector to enable the secretion of the major surface glycoprotein of HIV-1 (gp120) fused to the carboxy-terminus of the widely used affinity tag glutathione S -transferase. The secreted protein can be purified in a single step with the minimum of denaturation on immobilised glutathione and is as active as the parental molecule in binding CD4. We use...
Recombinant baculoviruses are widely used to express heterologous proteins. They are particularly useful for the high-level expression of eukaryotic proteins with fastidious co- or post-translational processing requirements. The recent publication of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome will greatly increase the sophistication of baculovirus molecular biology. Recently,...
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