Search results for: K. Akiyoshi

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article

11 Use of IHC and DISH of EGFR to evaluate efficacy of anti-EGFR drugs in KRAS-WT patients with mCRC

N. Takahashi, Y. Yamada, H. Taniguchi, K. Akiyoshi, more

European Journal of Cancer > 2012 > 48 > Supplement 2 > S15-S16

article

113. Comparing T-Spot and Tuberculosis Skin Testing Screening Methods for Treatment Acceptance and Cost Efficacy

Grace Kiet, Paul Holtom, Lawrence Neinstein, Tammie K. Akiyoshi, more

Journal of Adolescent Health > 2012 > 50 > 2 Supplement > S68

article

ACKNOWLEDGEMENT OF REVIEWERS

NG Adams, T Adekambi, J Afeltra, J Aguado, more

Clinical Microbiology and Infection > 2011 > 17 > 1 > 102-103

chapter

GFP synthesis in giant liposomes using the in vitro translation system of Thermococcus kodakaraensis

K. Yamaji, T. Kanai, S.-i.M. Nomura, K. Akiyoshi, more

2009 International Symposium on Micro-NanoMechatronics and Human Science > 176 - 181

2009 International Symposium on Micro-NanoMechatronics and Human Science (MHS)

The possibilities of an in vitro translation system, based on cell components of the hyperthermophilic archaeon, Thermococcus kodakaraensis, to utilize at a lower temperature range were examined. Green fluorescence protein (GFP) was used as the reporter protein. Through optimization of several experimental conditions, the yield of active GFP production at 40??C increased 5-fold. The optimized system...

article

Protein Synthesis in Giant Liposomes Using the <emphasis emphasistype="italic">In Vitro</emphasis> Translation System of <emphasis emphasistype="italic">Thermococcus kodakaraensis</emphasis>

K. Yamaji, T. Kanai, S.-I.M. Nomura, K. Akiyoshi, more

IEEE Transactions on NanoBioscience > 2009 > 8 > 4 > 325 - 331

An in vitro translation system, based on cell components of the hyperthermophilic archaeon, Thermococcus kodakaraensis, has previously been developed. The system has been optimized and applied for protein production at high temperatures (60-65??C). In this paper, we have examined the possibilities to utilize this system at a lower temperature range using green fluorescence protein (GFP) as the reporter...

article

Nanoparticle of cholesterol-bearing pullulan as a carrier of anticancer drugs

K. Satoh, F. Chen, A. Aoyama, H. Date, more

EJC Supplements > 2008 > 6 > 9 > 139

chapter

A Communication interface using vesicles embedded with channel forming proteins in molecular communication

Y. Moritani, S. Hiyama, S.M. Nomura, K. Akiyoshi, more

2007 2nd Bio-Inspired Models of Network, Information and Computing Systems > 147 - 149

2007 2nd Bio-Inspired Models of Network, Information and Computing Systems (BIONETICS)

This paper describes design and empirically study of a communication interface in molecular communication. The communication interface hides the characteristics of the molecules during the propagation from the sender to a receiver to allow a generic transport of molecules independent of the characteristics. The authors of this paper propose a communication interface that uses a vesicle embedded with...

chapter

Lipid-tubular network formation for biochemical reaction

S.M. Nomura, K. Akiyoshi

2007 International Symposium on Micro-NanoMechatronics and Human Science > 322 - 326

2007 18th International Symposium on Micro-NanoMechatronics and Human Science

Here we describe liposome connecting tubular networks as a chemical reactor system. Two types of cell-sized liposomes are prepared by natural swelling method. The one was encapsulating in vitro gene-expression system (without DNA) and the other was including plasmid DNA coding green fluorescent protein (GFP). After mixing them gently, ganglioside GM3 was added for the connecting liposomes to network...

chapter

Enzyme-Responsive Micelle-vesicle Transition: A New Method for the Reconstitution of Trans-membrane Protein to Liposome

K. Akiyoshi, N. Morimoto, A. Murota

2007 International Symposium on Micro-NanoMechatronics and Human Science > 91 - 95

2007 18th International Symposium on Micro-NanoMechatronics and Human Science

New enzyme-responsive micellar systems in which an enzymatic reaction controls the amphiphilicity of the surfactants were reported. The surfactants in our study consist of a short alkyl chain and a maltooligomer as a primer which can be synthesized enzymatically. In the presence of phosphorylase and alpha-D-glucose-1-phosphate (G1P), the elongation reaction of the saccharide chain proceeds from the...

chapter

A Molecular Communication Interface Using Liposomes with Gap Junction Proteins

Y. Moritani, S.M. Nomura, S. Hiyama, K. Akiyoshi, more

2006 1st Bio-Inspired Models of Network, Information and Computing Systems > 1

2006 First Bio-Inspired Models of Network, Information and Computing Systems

Molecular communication is an emerging communication paradigm that uses molecules as a communication medium. Molecular communication allows biological and artificially created nano- or cell-scale devices to communicate with each other. This paper proposes a molecular communication system that uses liposomes with gap junction proteins as an interface. A liposome acts as a container of information molecules,...

article

Single molecular mechanics of a cholesterol-bearing pullulan nanogel at the hydrophobic interfaces

ImShik Lee, K. Akiyoshi

Biomaterials > 2004 > 25 > 15 > 2911-2918

The study of nanogel (hydrogel nanoparticle) has intensified in the last decade due to the enormous potential applications in biomimetics, biosensors, artificial muscles and drug delivery (or release) systems. Cholesterol-bearing pullulan (CHP) is composed of hydrophilic pullulan backbone and partly substituted hydrophobic cholesterol, and is capable of forming a stable hydrogel nanoparticle in aqueous...

article

2P-0528 Common carotid artery intima-media thickness and ischemic stroke

K. Akiyoshi, S. Tetsunori, M. Hara, S. Nobe, more

Atherosclerosis (Supplements) (Component) > 2003 > 4 > 2 > 155

article

Single chip H.32X multimedia communication processor with CIF 30f/s MPEG4/H.26X bi-directional codec

N. Minegishi, N. Motoyama, M. Takagi, F. Ogawa, more

Proceedings of the 00027th European Solid-State Circuits Conference > 2001 > 129 - 132

Proceedings of the 27th European Solid-State Circuits Conference

A single multimedia communication chip suitable for plural products has been developed. This chip realizes real-time bi-directional encoding/decoding for CIF-resolution video at the frame rate of 30f/s, and meets H320, H.323, and H.324 standards. The chip is composed of a video-processing unit for H.26X standards, a DSP unit for speech codec and multiplex process, and a RISC unit for managing the...

article

Effect of TS-962, an ACAT inhibitor, on histological composition of aortic lesions in WHHL rabbit long-term atherosclerotic model

Y. Asami, H. Tomoike, K. Kaneko, S. Tanikawa, more

Atherosclerosis > 2000 > 151 > 1 > 119

article

Inhibitory effect of TS-962, an acat inhibitor, on intimal thickening of carotid artery in a rabbit balloon injury model

H. Tomoike, Y. Asami, I. Yamagishi, K. Akiyoshi, more

Atherosclerosis > 2000 > 151 > 1 > 108

article

Inhibitory effect of TS-962 on the formation of early atherosclerotic lesions in high fat-fed hyperlipidemic hamsters

Y. Asami, I. Yamagishi, K. Akiyoshi, H. Tomoike, more

Atherosclerosis > 1999 > 146 > 2 > 237 - 242

The hypocholesterolemic and anti-atherosclerotic effect of TS-962, a specific ACAT inhibitor, was investigated in a hamster model fed a high fat diet containing 10% coconut oil and 0.05% cholesterol. Lipid accumulated atherosclerotic lesions were detected by using oil red O staining in the lesion-prone aortic arch. A high dose, estimated to be 15 mg/kg, of TS-962 decreased serum cholesterol to normal...

article

Characterization of ancient Chinese pottery decorated with a black pigment

M Uda, K Akiyoshi, M Nakamura

Nuclear Inst. and Methods in Physics Research, B > 1999 > 150 > 1-4 > 601-604

The Yangshao type pottery, made about 6000 yrs ago, was investigated by X-ray diffraction (XRD), and confirmed to be composed of quartz, feldspar, muscovite and calcite. A black pigment on it was assumed to be (Mn, Fe) ₃ O ₄ from Particle Induced X-ray Emission (PIXE) and XRD experiments. Firing temperature of the pottery was assumed to be less than 600°C from a heating experiment...

article

Free Jejunal Graft Autotransplantation Should Be Revascularized Within 3 Hours

S. Hikida, M. Takeuchi, H. Hata, H. Yamana, more

Transplantation Proceedings > 1998 > 30 > 7 > 3446-3448

article

Abnormal ATP-stress Tl-SPECT imaging and poor coronary flow reserve in syndrome X

T. Akimitsu, K. Akiyoshi, K. Ito, K. Kaneda, more

Journal of Nuclear Cardiology > 1997 > 4 > 1 Part 2 > S86

article

Supramolecular assembly of hydrophobized polysaccharides

K. Akiyoshi, J. Sunamoto

Supramolecular Science > 1996 > 3 > 1-3 > 157-163

Hydrophobized polysaccharides, which were partly substituted by hydrophobes such as cholesterol, formed a hydrogel nanoparticle by their self-assembly in water. The size, density and colloidal stability of the nanoparticle were controlled by changing the substitution degree of hydrophobes and the hydrophobicity. The hydrophobized polysaccharides were further functionalized by conjugation of cell-specific...

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