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Prior strategies to measure inositol 1,4,5-trisphosphate (IP 3 ) in single cells either have been qualitative or have had a limited spatial resolution. Capillary electrophoresis combined with a biological detector cell has been used to quantitate IP 3 in small regions of a Xenopus oocyte. To improve the detection limits of this method, we elucidated the experimental parameters which...
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