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The ability to switch between a yeast-like form and a filamentous form is an extended characteristic among several fungi. In pathogenic fungi, this capacity has been correlated with virulence because along the infection process, dimorphic transitions are often required. Two well-known organisms for which dimorphism have been studied are the pathogenic fungi Candida albicans and Ustilago maydis, which...
The events that take place at the prokaryotic enhancer of the Pu promoter of Pseudomonas putida prior to the engagement of the σ 54 -RNA polymerase (σ 54 -RNAP) have been studied in vitro. ATP hydrolysis by XylR, the cognate regulator of the system, is preceded by the multimerization of XylR at the enhancer, which is itself triggered by the sole allosteric effect of ATP binding...
A simplified procedure to construct recombinant Pseudomonas putida (Pp) and related bacteria, which transcribe conditionally specific genes inserted into their chromosome in response to lac inducers such as IPTG, has been developed. The method is based on the so-called VTR expression cassettes. These are three small (1.98-kb) DNA segments engineered as NotI restriction fragments that include a lacI...
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