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We proposed a novel approach for monitoring cellular caspase-3 activities and controlling morphology of a cell under real time condition using fluorescence resonance energy transfer (FRET) based-bioprobes and the MEMS fabricated microculture dish. The FRET-based bioprobes monitoring caspase-3 activities were successfully introduced into the PC12 cells. In addition, the morphology of the bioprobes...
This paper presents microdroplet merging device which can actively control the number of merged microdroplets with a wide range of volume under various flow conditions. Independently generated two different droplets are trapped at pillar structure and its trapping time is controlled by horizontal pneumatic micro actuators. The droplet merging of multi-mode from no-merging to 4 droplets merging is...
Au deposited SU-8 microdots comprised SU-8-PET microchip using low temperature polymer bonding technology is developed. A fine microdot array (1µm×1µm×0.5µm dots with 2µm pitch in area of 50µm×5mm) is fabricated by electron beam lithography (EBL) using SU-8 as a negative tone resist. A microchannel structure is formed with UV patterned SU-8 on a glass substrate. The SU-8 structure is sealed with a...
MEMS liquid chromatography (LC) microchip with low dispersion and a low pressure drop turn structure was fabricated. In the turn structure, we used higher density pillar array at the inner side while lower density pillar array was used at the outer side to control inner and outer speed of a sample flow. An LC chip with an improved turn structure can obtain similar theoretical plate number (1233) compared...
This paper proposes a high accuracy fabrication method for multilayer PDMS (polydimethylsiloxane) devices. Based on newly developed multi-stacking process using three-dimensional (3D) alignment marks of the concave-convex pair structures, a multilayer PDMS device was fabricated without skillful handling for alignment. Total misalignment of smaller than 10 μm is achieved for fabrication of a five-layer...
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