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A recombinant form of Plasmodium falciparum β-ketoacyl-ACP reductase (PfFabG) was overexpressed in Escherichia coli BL-21 codon plus (DE3). The resulting insoluble inclusion bodies were separated from cellular debris by extensive washing with buffer containing 0.05% Tween 20 and solubilized by homogenization with 8M urea. Attempts to refold PfFabG from solubilized inclusion bodies employing Rotofor...