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A polyclonal antibody against the Na + ,K + -ATPase holoenzyme was prepared. This antibody recognized native Na + ,K + -ATPase and inhibited its activity. The peptide fragments corresponding to various regions of the Na + ,K + -ATPase α-subunit, which were synthesized from the cDNA, were immunoprecipitated with the antibody, and the M32-D75, M158-D197...