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Saponin-rich GM extract was prepared by refluxing 25g of GM with 250ml of EtOH/H 2 O (1:1, v/v) for 3h then filtering and distilling EtOH at 50°C. The refluxed extract was partitioned with equal volume of BuOH obtaining crude saponin-rich GM extract with 4.8±0.6% DM of GM that was purified by RP-HPLC eluting 20%, 60% and 100% MeOH fractions with 2.04±0.32%, 0.91±0.16% and 1.55±0.15% DM of...
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