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The first centromeric protein identified in any species was CENP-A, a divergent member of the histone H3 family that was recognised by autoantibodies from patients with scleroderma-spectrum disease. It has recently been suggested to rename this protein CenH3. Here, we argue that the original name should be maintained both because it is the basis of a long established nomenclature for centromere proteins...
When using deformable models for the segmentation of biological data, the choice of the best weighting parameters for the internal and external forces is crucial. Especially when dealing with 3D fluorescence microscopic data and cells within dense tissue, object boundaries are sometimes not visible. In these cases, one weighting parameter set for the whole contour is not desirable. We are presenting...
Gradient vector flow snakes are a very common method in bio-medical image segmentation. The use of gradient vector flow herein brings some major advantages like a large capture range and a good adaption of the snakes in concave regions. In some cases though, the application of gradient vector flow can also have undesired effects, e.g. if only parts of an image are strongly blurred, the remaining weak...
We describe the preparation of nuclear extracts from yeast cells synchronised in S-phase that support the aphidicolin-sensitive, semi-conservative replication of primer-free, supercoiled plasmid in vitro. This is monitored by one and two-dimensional gel electrophoresis of replication intermediates that have incorporated [α-32P]dATP, by the conversion of methylated template DNA into a hemi-methylated...
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