The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
We demonstrated a photothermal delivery approach with self-aligned cell seeding, which was realized by simple fabrication of microwells and sharp tips. Calcein molecules (622 Da) were successfully delivered into cells with above 80% viability.
We report a novel sheathless microfluidic fluorescence-activated cell sorter utilizing size-independent three-dimensional dielectrophoretic (DEP) single stream focusing and pulsed laser activated cell sorting (PLACS). This is realized by fabricating a 3D microfluidic device using two glass substrates with patterned electrodes sandwiching a thin and open PDMS channel. DEP forces are provided along...
Technologies for transferring large-sized cargo into mammalian cells are needed to advance key applications in cell engineering. However, reliable methodologies for introducing large-sized cargo into mammalian cells are nearly completely lacking. This talk will present two new technologies, photothermal nanoblade and BLAST, that overcome the size limitation of cargo delivery into mammalian cells.
Cells form the basic unit of life. Their health and activities can be quantified by a multitude of biochemical and biophysical techniques that measure responses to external or internal stimuli. Many experimental approaches attempt to integrate molecular mechanisms with changes in the mechanical properties of cells, such as visocoelasticity and compliance, to link cell function with structure. An emerging...
We present a Pulsed Laser Activated Cell Sorter (PLACS) integrated with 3D sheathless inertial focusing that is capable to sort at 10,000 particles sec−1 with >90% sort purity and 6,000 cells sec−1 with >80% sort purity. It is realized by exciting laser induced cavitation bubbles in a single layer PDMS microfluidic channel to create high speed liquid jets to deflect detected fluorescent samples...
We demonstrated direct nuclear delivery of macromolecules into live mammalian cells using the photothermal nanoblade. Pulsed laser triggered cavitation bubbles on a titanium thin film coated micropipette tip was utilized to puncture both the cell plasma and nuclear membranes followed by pressure controlled delivery of cargo into the nucleus. High plasmid expression (79–100%) in different cell types...
We report a 3D PDMS microfluidic pulsed laser triggered fluorescence activated cell sorter capable of sorting at 11,000 cells sec−1 with >95% purity or at 45,000 cells sec−1 with 45% purity within a single channel.
We present a novel electrical impedance sensor for measuring the resealing time of porated mammalian plasma membranes. Openings were generated by high speed cavitation bubbles using laser pulsing of metallic microdisks on transparent ITO electrodes. Real-time impedance measurements show that membrane resealing time and impedance recovery takes 1 to 2 minutes after laser pulsing. Our method can also...
We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy to generate highly localized explosive vapor bubbles for rapid cell membrane cutting. The cavitation bubble pattern is controlled by the metallic structure configuration, laser pulse duration and energy. Integrating the metallic nanostructure with a micropipette, the nanoblade can generate...
Cancer and many other diseases are characterized by changes in cell morphology, motion and mechanical rigidity. However, in live-cell-cytology stimulus-induced morphologic changes typically take 10–30 minutes to detect. Here, we employ live-cell-interferometry (LCI) to visualize the instantaneous response of a whole cell to mechanical stimulation, and we detect cytoskeletal remodeling behavior within...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.