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We developed a rapid fluorometric viability assay for primary cultures of OL precursors (preOLs) or mature OLs that utilized the oxidation/reduction indicator dye Alamar Blue (AB). PreOLs had a lower rate of AB reduction than did mature OLs (0.02+/-0.01 units/min per cell versus 0.07+/-0.01). The assay was tested under two conditions toxic to preOLs: oxidative stress induced by glutathione depletion...