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To improve the fermentation potency of phytase in recombinant hosts and decrease the production cost, the Pichia expression vector pGAPZα-A was modified by introduction of an AOX1 promoter to generate the plasmid, pAOXZα. A phytase gene appA-m was cloned into pAOXZα to construct a methanol-induced expression vector pAOXZα-appA-m. The recombinant Pichia pastoris 74 # , which contained one copy...