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Human sleep is a natural part of every individual's life. Clear relationship between sleep and endocrine system has been already established. In particular, melatonin and cortisol are known to affect and regulate sleep/wake patterns. Here we report the development of an ultra-high pressure liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for simultaneous measurement of 6-sulfatoxymelatonin...