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Monitoring the lineage-specific chimerism following stem cell transplantation is a valuable tool in detection of relapse and directing post-transplant treatment. Assessment of chimerism of multiple lineages is becoming a routine practice and may pose a problem when a limited amount of blood can be collected from a patient, especially a young child. In this case, the first round of positive selection...
Commercial cell separation kits based on monoclonal antibodies (mAb) against CD markers are widely used for monitoring lineage-specific chimerism testing.We use the myeloid cell kit by StemCell Technologies for CD33 and CD66b positive selection (clones D34260.251 and BIRMA17C).Patient received a related stem cell transplant and engraftment monitoring was done monthly on blood, CD3 and myeloid cell...