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We developed a TaqMan real-time polymerase chain reaction (PCR) assay for the quantitative detection of Theileria equi from the in vitro-cultured parasite and field blood samples collected from horses living in Ghana and Brazil. The detection limit for the assay was determined to be 1.5parasites/μl per sample, and the quantitative capacity was demonstrated using the in vitro-cultured parasite. For...