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Denatured and fully reduced lysozyme and ribonuclease A were refolded in batch and also in fed-batch operation. In fed-batch refolding of lysozyme, denatured lysozyme was gradually added into refolding buffers containing urea in the concentration range from 1.0 to 2.0 mol/l. The recoveries of the lysozyme activity in a fed-batch operation were higher than those in a batch operation. In the case of...