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Serum specimens from 58 hospitalized patients with clinical suspicion of dengue fever were obtained in a prospective study. All 58 acute serum samples were tested by IgM capture enzyme-linked immunosorbent assay (ELISA) and commercial Dengue Blot serological assays, virus isolation in C6 mosquito cells, and polymerase chain reaction (PCR) by single-step and semi-nested approaches using NS3 gene primers...